Bioinformatics and molecular approaches to detect NRPS genes involved in the biosynthesis of kurstakin from Bacillus thuringiensis.
Identifieur interne : 000131 ( Main/Exploration ); précédent : 000130; suivant : 000132Bioinformatics and molecular approaches to detect NRPS genes involved in the biosynthesis of kurstakin from Bacillus thuringiensis.
Auteurs : Ahmed Abderrahmani [France] ; Arthur Tapi ; Farida Nateche ; Marlène Chollet ; Valérie Leclère ; Bernard Wathelet ; Hocine Hacene ; Philippe JacquesSource :
- Applied microbiology and biotechnology [ 1432-0614 ] ; 2011.
Descripteurs français
- KwdFr :
- Algérie (MeSH), Amino-acid ligases (génétique), Amino-acid ligases (métabolisme), Amorces ADN (MeSH), Bacillus cereus (génétique), Bacillus thuringiensis (composition chimique), Bacillus thuringiensis (génétique), Bacillus thuringiensis (isolement et purification), Bacillus thuringiensis (métabolisme), Banque de gènes (MeSH), Biologie informatique (méthodes), Lipopeptides (biosynthèse), Opéron (MeSH), Spectrométrie de masse MALDI (MeSH), Voies de biosynthèse (génétique).
- MESH :
- biosynthèse : Lipopeptides.
- composition chimique : Bacillus thuringiensis.
- génétique : Amino-acid ligases, Bacillus cereus, Bacillus thuringiensis, Voies de biosynthèse.
- isolement et purification : Bacillus thuringiensis.
- métabolisme : Amino-acid ligases, Bacillus thuringiensis.
- méthodes : Biologie informatique.
- Algérie, Amorces ADN, Banque de gènes, Opéron, Spectrométrie de masse MALDI.
- Wicri :
- geographic : Algérie.
English descriptors
- KwdEn :
- Algeria (MeSH), Bacillus cereus (genetics), Bacillus thuringiensis (chemistry), Bacillus thuringiensis (genetics), Bacillus thuringiensis (isolation & purification), Bacillus thuringiensis (metabolism), Biosynthetic Pathways (genetics), Computational Biology (methods), DNA Primers (MeSH), Gene Library (MeSH), Lipopeptides (biosynthesis), Operon (MeSH), Peptide Synthases (genetics), Peptide Synthases (metabolism), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH).
- MESH :
- chemical , biosynthesis : Lipopeptides.
- chemical , genetics : Peptide Synthases.
- chemical , metabolism : Peptide Synthases.
- chemical : DNA Primers.
- geographic : Algeria.
- chemistry : Bacillus thuringiensis.
- genetics : Bacillus cereus, Bacillus thuringiensis, Biosynthetic Pathways.
- isolation & purification : Bacillus thuringiensis.
- metabolism : Bacillus thuringiensis.
- methods : Computational Biology.
- Gene Library, Operon, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
Abstract
Degenerated primers designed for the detection by polymerase chain reaction of nonribosomal peptide synthetases (NRPS) genes involved in the biosynthesis of lipopeptides were used on genomic DNA from a new isolate of Bacillus thuringiensis CIP 110220. Primers dedicated to surfactin and bacillomycin detection amplified sequences corresponding respectively to the surfactin synthetase operon and to a gene belonging to a new NRPS operon identified in the genome of B. thuringiensis serovar pondicheriensis BSCG 4BA1. A bioinformatics analysis of this operon led to the prediction of an NRPS constituted of seven modules beginning with a condensation starter domain and which could be involved in the biosynthesis of a heptalipopeptide similar to kurstakin. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS) performed on whole cells of B. thuringiensis CIP 110220 confirmed the production of kurstakin by this strain. The kurstakin operon was thus used to design a new set of degenerated primers specifically to detect kurstakin genes. These primers were used to screen kurstakin producers in a collection of nine B. thuringiensis strains isolated from different areas in Algeria and two from the Pasteur Institute collection. For eight among the 11 tested strains, the amplified fragment matched with an operon similar to the kurstakin operon and found in the newly sequenced genome of Bacillus cereus or B. thuringiensis serovar pulsiensis, kurstaki, and thuringiensis. Kurstakin production was detected by MALDI-ToF-MS on whole cells for six strains. This production was compared with the spreading of the strains and their antimicrobial activity. Only the spreading can be correlated with the kurstakin production.
DOI: 10.1007/s00253-011-3453-6
PubMed: 21751008
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000692
- to stream PubMed, to step Curation: 000688
- to stream PubMed, to step Checkpoint: 000695
- to stream Main, to step Merge: 000131
- to stream Main, to step Curation: 000131
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Bioinformatics and molecular approaches to detect NRPS genes involved in the biosynthesis of kurstakin from Bacillus thuringiensis.</title><author><name sortKey="Abderrahmani, Ahmed" sort="Abderrahmani, Ahmed" uniqKey="Abderrahmani A" first="Ahmed" last="Abderrahmani">Ahmed Abderrahmani</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire des Procédés Biologiques, Génie Enzymatique et Microbien, ProBioGEM, UPRES-EA 1026, Polytech'Lille/IUT A, Université Lille Nord de France-Sciences et Technologies, USTL, Avenue Paul Langevin, 59655, Villeneuve d'Ascq Cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire des Procédés Biologiques, Génie Enzymatique et Microbien, ProBioGEM, UPRES-EA 1026, Polytech'Lille/IUT A, Université Lille Nord de France-Sciences et Technologies, USTL, Avenue Paul Langevin, 59655, Villeneuve d'Ascq Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Hauts-de-France</region><region type="old region" nuts="2">Nord-Pas-de-Calais</region><settlement type="city">Villeneuve d'Ascq</settlement></placeName></affiliation></author><author><name sortKey="Tapi, Arthur" sort="Tapi, Arthur" uniqKey="Tapi A" first="Arthur" last="Tapi">Arthur Tapi</name></author><author><name sortKey="Nateche, Farida" sort="Nateche, Farida" uniqKey="Nateche F" first="Farida" last="Nateche">Farida Nateche</name></author><author><name sortKey="Chollet, Marlene" sort="Chollet, Marlene" uniqKey="Chollet M" first="Marlène" last="Chollet">Marlène Chollet</name></author><author><name sortKey="Leclere, Valerie" sort="Leclere, Valerie" uniqKey="Leclere V" first="Valérie" last="Leclère">Valérie Leclère</name></author><author><name sortKey="Wathelet, Bernard" sort="Wathelet, Bernard" uniqKey="Wathelet B" first="Bernard" last="Wathelet">Bernard Wathelet</name></author><author><name sortKey="Hacene, Hocine" sort="Hacene, Hocine" uniqKey="Hacene H" first="Hocine" last="Hacene">Hocine Hacene</name></author><author><name sortKey="Jacques, Philippe" sort="Jacques, Philippe" uniqKey="Jacques P" first="Philippe" last="Jacques">Philippe Jacques</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21751008</idno><idno type="pmid">21751008</idno><idno type="doi">10.1007/s00253-011-3453-6</idno><idno type="wicri:Area/PubMed/Corpus">000692</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000692</idno><idno type="wicri:Area/PubMed/Curation">000688</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000688</idno><idno type="wicri:Area/PubMed/Checkpoint">000695</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000695</idno><idno type="wicri:Area/Main/Merge">000131</idno><idno type="wicri:Area/Main/Curation">000131</idno><idno type="wicri:Area/Main/Exploration">000131</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Bioinformatics and molecular approaches to detect NRPS genes involved in the biosynthesis of kurstakin from Bacillus thuringiensis.</title><author><name sortKey="Abderrahmani, Ahmed" sort="Abderrahmani, Ahmed" uniqKey="Abderrahmani A" first="Ahmed" last="Abderrahmani">Ahmed Abderrahmani</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire des Procédés Biologiques, Génie Enzymatique et Microbien, ProBioGEM, UPRES-EA 1026, Polytech'Lille/IUT A, Université Lille Nord de France-Sciences et Technologies, USTL, Avenue Paul Langevin, 59655, Villeneuve d'Ascq Cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire des Procédés Biologiques, Génie Enzymatique et Microbien, ProBioGEM, UPRES-EA 1026, Polytech'Lille/IUT A, Université Lille Nord de France-Sciences et Technologies, USTL, Avenue Paul Langevin, 59655, Villeneuve d'Ascq Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Hauts-de-France</region><region type="old region" nuts="2">Nord-Pas-de-Calais</region><settlement type="city">Villeneuve d'Ascq</settlement></placeName></affiliation></author><author><name sortKey="Tapi, Arthur" sort="Tapi, Arthur" uniqKey="Tapi A" first="Arthur" last="Tapi">Arthur Tapi</name></author><author><name sortKey="Nateche, Farida" sort="Nateche, Farida" uniqKey="Nateche F" first="Farida" last="Nateche">Farida Nateche</name></author><author><name sortKey="Chollet, Marlene" sort="Chollet, Marlene" uniqKey="Chollet M" first="Marlène" last="Chollet">Marlène Chollet</name></author><author><name sortKey="Leclere, Valerie" sort="Leclere, Valerie" uniqKey="Leclere V" first="Valérie" last="Leclère">Valérie Leclère</name></author><author><name sortKey="Wathelet, Bernard" sort="Wathelet, Bernard" uniqKey="Wathelet B" first="Bernard" last="Wathelet">Bernard Wathelet</name></author><author><name sortKey="Hacene, Hocine" sort="Hacene, Hocine" uniqKey="Hacene H" first="Hocine" last="Hacene">Hocine Hacene</name></author><author><name sortKey="Jacques, Philippe" sort="Jacques, Philippe" uniqKey="Jacques P" first="Philippe" last="Jacques">Philippe Jacques</name></author></analytic><series><title level="j">Applied microbiology and biotechnology</title><idno type="eISSN">1432-0614</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Algeria (MeSH)</term><term>Bacillus cereus (genetics)</term><term>Bacillus thuringiensis (chemistry)</term><term>Bacillus thuringiensis (genetics)</term><term>Bacillus thuringiensis (isolation & purification)</term><term>Bacillus thuringiensis (metabolism)</term><term>Biosynthetic Pathways (genetics)</term><term>Computational Biology (methods)</term><term>DNA Primers (MeSH)</term><term>Gene Library (MeSH)</term><term>Lipopeptides (biosynthesis)</term><term>Operon (MeSH)</term><term>Peptide Synthases (genetics)</term><term>Peptide Synthases (metabolism)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Algérie (MeSH)</term><term>Amino-acid ligases (génétique)</term><term>Amino-acid ligases (métabolisme)</term><term>Amorces ADN (MeSH)</term><term>Bacillus cereus (génétique)</term><term>Bacillus thuringiensis (composition chimique)</term><term>Bacillus thuringiensis (génétique)</term><term>Bacillus thuringiensis (isolement et purification)</term><term>Bacillus thuringiensis (métabolisme)</term><term>Banque de gènes (MeSH)</term><term>Biologie informatique (méthodes)</term><term>Lipopeptides (biosynthèse)</term><term>Opéron (MeSH)</term><term>Spectrométrie de masse MALDI (MeSH)</term><term>Voies de biosynthèse (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Lipopeptides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peptide Synthases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Peptide Synthases</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Algeria</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Lipopeptides</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Bacillus thuringiensis</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Bacillus thuringiensis</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacillus cereus</term><term>Bacillus thuringiensis</term><term>Biosynthetic Pathways</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Amino-acid ligases</term><term>Bacillus cereus</term><term>Bacillus thuringiensis</term><term>Voies de biosynthèse</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacillus thuringiensis</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Bacillus thuringiensis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacillus thuringiensis</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Computational Biology</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Amino-acid ligases</term><term>Bacillus thuringiensis</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Biologie informatique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Library</term><term>Operon</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Algérie</term><term>Amorces ADN</term><term>Banque de gènes</term><term>Opéron</term><term>Spectrométrie de masse MALDI</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Algérie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Degenerated primers designed for the detection by polymerase chain reaction of nonribosomal peptide synthetases (NRPS) genes involved in the biosynthesis of lipopeptides were used on genomic DNA from a new isolate of Bacillus thuringiensis CIP 110220. Primers dedicated to surfactin and bacillomycin detection amplified sequences corresponding respectively to the surfactin synthetase operon and to a gene belonging to a new NRPS operon identified in the genome of B. thuringiensis serovar pondicheriensis BSCG 4BA1. A bioinformatics analysis of this operon led to the prediction of an NRPS constituted of seven modules beginning with a condensation starter domain and which could be involved in the biosynthesis of a heptalipopeptide similar to kurstakin. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS) performed on whole cells of B. thuringiensis CIP 110220 confirmed the production of kurstakin by this strain. The kurstakin operon was thus used to design a new set of degenerated primers specifically to detect kurstakin genes. These primers were used to screen kurstakin producers in a collection of nine B. thuringiensis strains isolated from different areas in Algeria and two from the Pasteur Institute collection. For eight among the 11 tested strains, the amplified fragment matched with an operon similar to the kurstakin operon and found in the newly sequenced genome of Bacillus cereus or B. thuringiensis serovar pulsiensis, kurstaki, and thuringiensis. Kurstakin production was detected by MALDI-ToF-MS on whole cells for six strains. This production was compared with the spreading of the strains and their antimicrobial activity. Only the spreading can be correlated with the kurstakin production.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Hauts-de-France</li><li>Nord-Pas-de-Calais</li></region><settlement><li>Villeneuve d'Ascq</li></settlement></list><tree><noCountry><name sortKey="Chollet, Marlene" sort="Chollet, Marlene" uniqKey="Chollet M" first="Marlène" last="Chollet">Marlène Chollet</name><name sortKey="Hacene, Hocine" sort="Hacene, Hocine" uniqKey="Hacene H" first="Hocine" last="Hacene">Hocine Hacene</name><name sortKey="Jacques, Philippe" sort="Jacques, Philippe" uniqKey="Jacques P" first="Philippe" last="Jacques">Philippe Jacques</name><name sortKey="Leclere, Valerie" sort="Leclere, Valerie" uniqKey="Leclere V" first="Valérie" last="Leclère">Valérie Leclère</name><name sortKey="Nateche, Farida" sort="Nateche, Farida" uniqKey="Nateche F" first="Farida" last="Nateche">Farida Nateche</name><name sortKey="Tapi, Arthur" sort="Tapi, Arthur" uniqKey="Tapi A" first="Arthur" last="Tapi">Arthur Tapi</name><name sortKey="Wathelet, Bernard" sort="Wathelet, Bernard" uniqKey="Wathelet B" first="Bernard" last="Wathelet">Bernard Wathelet</name></noCountry><country name="France"><region name="Hauts-de-France"><name sortKey="Abderrahmani, Ahmed" sort="Abderrahmani, Ahmed" uniqKey="Abderrahmani A" first="Ahmed" last="Abderrahmani">Ahmed Abderrahmani</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/MaghrebDataLibMedV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000131 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000131 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= MaghrebDataLibMedV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:21751008
|texte= Bioinformatics and molecular approaches to detect NRPS genes involved in the biosynthesis of kurstakin from Bacillus thuringiensis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21751008" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MaghrebDataLibMedV2
| This area was generated with Dilib version V0.6.38. |  |